domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot
reactivity: mouse, rat
application: western blot
Western blot analysis of VEGF expression in rat kidney extract (lane 1). VEGF at 27KD was detected using rabbit anti-VEGF Antigen Affinity purified polyclonal antibody (Catalog # A00045) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
domestic rabbit monoclonal (ADB-22)
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Immunohistochemical analysis of paraffin-embedded human kindey, using VEGF Antibody(M00045-1). VEGFA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGFA Antibody (M00045-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunofluorescent analysis of HUVEC cells, using VEGF Antibody.
Flow cytometric analysis of Hela cells with VEGFantibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA
reactivity: mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates,. Lane 2: mouse HEPA1-6 whole cell lysates,. Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27KD, 40KD. The expected band size for VEGF is at 25KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: mouse
application: western blot, ELISA
reactivity: mouse
application: western blot, ELISA