Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)
Boster
catalog: MA1085

mouse monoclonal (PKB-175)
reactivity: human, mouse, rat, chicken
application: western blot

Anti- PKB alpha antibody, MA1085, Western blotting. All lanes: Anti PKB alpha (MA1085) at 0.5ug/ml. Lane 1: Rat Ovary Tissue Lysate at 50ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 56KD. Observed bind size: 56KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody
Boster
catalog: P00024-5

domestic rabbit monoclonal (IFD-1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody (P00024-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-5) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1

Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody(P00024-5). AKT1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (P00024-5)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-AKT1 Monoclonal Antibody
Boster
catalog: M00024-1

domestic rabbit monoclonal (EBG-1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunofluorescent analysis of Hela cells, using AKT1 Antibody.

Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (M00024-1). AKT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (M00024-1) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified polyclonal antibody (Catalog # M00024-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for AKT1.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Boster
catalog: P00024-2

domestic rabbit monoclonal (HEA-1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

Western blot analysis of Phospho-AKT1 (T450) expression in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate (P00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody
Boster
catalog: P00024-3

domestic rabbit monoclonal (HGD-1)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate (P00024-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-3) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Boster
catalog: P00024-4

domestic rabbit monoclonal (HIA-1)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate (P00024-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-4) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-AKT1,2,3 Antibody Picoband™
Boster
catalog: A00024-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
Boster
catalog: M00024-5

domestic rabbit monoclonal (17A20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 µl/vial
price: 315 USD
to the supplier

Anti-AKT1/2 Rabbit Monoclonal Antibody
Boster
catalog: M00024-6

domestic rabbit monoclonal (17A25)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 µl/vial
price: 315 USD
to the supplier