domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of Emerin using anti-Emerin antibody (A00714). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates,. Lane 2: mouse cardiac muscle tissue lysates,. Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Emerin antigen affinity purified polyclonal antibody (Catalog # A00714) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Emerin at approximately 34KD. The expected band size for Emerin is at 29KD.
Figure 2. IHC analysis of Emerin using anti-Emerin antibody (A00714). Emerin was detected in paraffin-embedded section of human endometrial carcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Emerin Antibody (A00714) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Emerin using anti-Emerin antibody (A00714). Emerin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Emerin Antibody (A00714) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (5A10)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
Figure 1. Western blot analysis of Emerin using anti-Emerin antibody (M00714). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human placenta tissue lysates, . Lane 3: human Caco-2 whole cell lysates, . Lane 4: human HepG2 whole cell lysates,. Lane 5: Rabbit IgG,. Lane 6: Marker 1113, . Lane 7: human Jurkat whole cell lysates. Lane 8: human MDA-MB-453 whole cell lysates, . Lane 9: human SK-OV-3 whole cell lysates,. Lane 10: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Emerin antigen affinity purified monoclonal antibody (Catalog # M00714) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Figure 2. IHC analysis of Emerin using anti-Emerin antibody (M00714). Emerin was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Emerin Antibody (M00714) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis of Emerin using anti-Emerin antibody (M00714). Emerin was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Emerin Antibody (M00714) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Human Emerin DyLight® 488 conjugated EMD Antibody(monoclonal, 5A10)
Boster
catalog: M00714-Dyl488
Boster
catalog: M00714-Dyl488
mouse monoclonal (5A10)
reactivity: human
conjugate: DyLight488
application: flow cytometry
reactivity: human
conjugate: DyLight488
application: flow cytometry
Anti-Human Emerin DyLight® 550 conjugated EMD Antibody(monoclonal, 5A10)
Boster
catalog: M00714-Dyl550
Boster
catalog: M00714-Dyl550
mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
domestic rabbit monoclonal (ACGA-5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry