Anti-EGFR Antibody Picoband™
Boster
catalog: A00023

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (A00023). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,. Lane 2: mouse brain tissue lysate,. Lane 3: human HepG2 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # A00023) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 175KD. The expected band size for EGFR is at 134KD.

Figure 3. IHC analysis of EGFR using anti-EGFR antibody (A00023). EGFR was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (A00023) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 315 USD
to the supplier

Anti-EGFR Antibody Picoband™
Boster
catalog: PB9016

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry

Anti-EGFR Picoband antibody, PB9016-1.jpg. All lanes: Anti-EGFR(PB9016) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: A431 Whole Cell Lysate at 40ug. Predicted bind size: 134KD. Observed bind size: 200KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody
Boster
catalog: P00023-2

domestic rabbit monoclonal (EHG-5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-EGFR (Y1173) expression in A431 cell lysate treated with EGF (P00023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-EGFR (Y1068) Rabbit Monoclonal Antibody
Boster
catalog: P00023-3

domestic rabbit monoclonal (HOF-5)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Phospho-EGFR (Y1068) expression in (1) A431 cell lysate; (2) A431 cell treated with EGF lysate (P00023-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-3) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-EGFR (Y1092) Rabbit Monoclonal Antibody
Boster
catalog: P00023

domestic rabbit monoclonal (BFO-5)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-EGFR (Y1092) Antibody (P00023). EGFR was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (P00023)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of EGFR phosphorylation expression in Huvec cell lysate treated with EGF (P00023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
quantity: 100 µl
price: 315 USD
to the supplier

Anti-EGFR (ErbB 1) Monoclonal Antibody
Boster
catalog: M00023-2

domestic rabbit monoclonal (AEF-5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Figure 2. IHC analysis of EGFR using anti-EGFR antibody (M00023-2)on paraffin-embedded human stomach cancer. EGFR was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (M00023-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (M00023-2) in HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # M00023-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for EGFR.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-EGFR (Y1086) Rabbit Monoclonal Antibody
Boster
catalog: P00023-1

domestic rabbit monoclonal (EFI-5)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-EGFR (Y1086) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with EGF (P00023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody
Boster
catalog: P00023-4

domestic rabbit monoclonal (GCC-5)
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of Phospho-EGFR (S695) expression in A431 cell lysate treated with EGF (P00023-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-4) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
quantity: 100 µl
price: 315 USD
to the supplier

Anti-EGFR Antibody
Boster
catalog: A00023-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunoprecipitation

quantity: 250 uL
price: 487 USD
to the supplier

Anti-EGFR Antibody Picoband™
Boster
catalog: A00023-4

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-EGFR Antibody Picoband™
Boster
catalog: A00023-5

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Phospho-EGFR (S1026) Antibody
Boster
catalog: A00023S1026

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (phospho-S1046) Antibody
Boster
catalog: A00023S1046

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 547 USD
to the supplier

Anti-EGFR (phospho-S1047) Antibody
Boster
catalog: A00023S1047

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot

quantity: 100 ul
price: 547 USD
to the supplier

Anti-EGFR (S1064) Antibody
Boster
catalog: A00023S1064

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-Phospho-EGFR (S1070) Antibody
Boster
catalog: A00023S1070

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (S1071) Antibody
Boster
catalog: A00023S1071

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (S1104) Antibody
Boster
catalog: A00023S1104

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot

quantity: 100 ul
price: 512 USD
to the supplier

Anti-EGFR (Phospho-S695) Antibody
Boster
catalog: A00023S695

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-Phospho-EGFR (S695) Antibody
Boster
catalog: A00023S695-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (T1191) Antibody
Boster
catalog: A00023T1191

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-EGFR (phospho-T669) Antibody
Boster
catalog: A00023T669-1

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 547 USD
to the supplier

Anti-Phospho-EGFR (T678) Antibody
Boster
catalog: A00023T678

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (T693) Antibody
Boster
catalog: A00023T693

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (Y1016) Antibody
Boster
catalog: A00023Y1016

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (Phospho-Y1068) Antibody
Boster
catalog: A00023Y1068-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-Phospho-EGFR (Y1069) Antibody
Boster
catalog: A00023Y1069

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (Phospho-Y1092) Antibody
Boster
catalog: A00023Y1092

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-Phospho-EGFR (Y1092) Antibody
Boster
catalog: A00023Y1092-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (Y1110) Antibody
Boster
catalog: A00023Y1110

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (Y1172) Antibody
Boster
catalog: A00023Y1172

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (Phospho-Y1173) Antibody
Boster
catalog: A00023Y1173

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ul
price: 697 USD
to the supplier

Anti-Phospho-EGFR (Y1197) Antibody
Boster
catalog: A00023Y1197

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-EGFR (Y869) Antibody
Boster
catalog: A00023Y869

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (phospho-Y992) Antibody
Boster
catalog: A00023Y992-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 547 USD
to the supplier

Anti-EGFR (Phospho-Tyr998) Antibody
Boster
catalog: A00023Y998-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody
Boster
catalog: M00023

mouse monoclonal (GFR450)
reactivity: human
application: flow cytometry

quantity: 100 ug/vial
price: 440 USD
to the supplier

Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294
Boster
catalog: M00023-3

domestic rabbit monoclonal (RM294)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier

Anti-EGFR Rabbit Monoclonal Antibody
Boster
catalog: M00023-4

domestic rabbit monoclonal (1.7E+17)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 µl/vial
price: 315 USD
to the supplier

Anti-EGFR(L858R mutation) Rabbit Monoclonal Antibody
Boster
catalog: M00023-5

domestic rabbit monoclonal (2E+89)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 µl/vial
price: 315 USD
to the supplier

Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269
Boster
catalog: P00023-5

domestic rabbit monoclonal (RM269)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier