domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1
Figure 1. Western blot analysis of Nos2 using anti-Nos2 antibody (A00368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Nos2.
quantity: 100µl
price: 563 USD
to the supplier
domestic rabbit monoclonal (K13-A)
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunoprecipitation
Figure 1. IHC analysis of NOS2 using anti-NOS2 antibody (M00368-1). NOS2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOS2 Antibody (M00368-1) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µl
price: 656 USD
to the supplier
domestic rabbit monoclonal (IED-14)
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of iNOS expression in Human fetal brain lysate (M00368). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS2 monoclonal antibody (Catalog # M00368) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS2
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA
reactivity: mouse
application: western blot, ELISA