domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: mouse, rat
application: western blot, ELISA, immunohistochemistry
Figure 1. Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, . Lane 2: mouse kidney tissue lysates,. Lane 3: mouse testis tissue lysates,. Lane 4: mouse ovary tissue lysates,. Lane 5: rat heart tissue lysates,. Lane 6: rat skeletal muscle tissue lysates,. Lane 7: rat gaster tissue lysates, . Lane 8: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Figure 2. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Galectin 1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin 1 Antibody (A00470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Galectin 1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin 1 Antibody (A00470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Galectin 1 expression in rat testis extract (lane 1), mouse spleen extract (lane 2), HELA whole cell lysates (lane 3) and SMMC whole cell lysates (lane 4). Galectin 1 at 15KD was detected using rabbit anti- Galectin 1 Antigen Affinity purified polyclonal antibody (Catalog # PB9240-1) at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Galectin 1 was detected in paraffin-embedded sections of mouse intestine cancer tissues using rabbit anti- Galectin 1 Antigen Affinity purified polyclonal antibody (Catalog # PB9240-1) at 1 ??g/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Galectin 1 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Galectin 1 Antigen Affinity purified polyclonal antibody (Catalog # PB9240-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA2010).
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Figure 1. Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, . Lane 2: rat ovary tissue lysates,. Lane 3: rat testis tissue lysates,. Lane 4: mouse kidney tissue lysates,. Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470-1) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Figure 2. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470-1). Galectin 1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin 1 Antibody (A00470-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470-1). Galectin 1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin 1 Antibody (A00470-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
