domestic rabbit monoclonal (BDF-4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody(M01948-1). DES was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DES Antibody (M01948-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Desmin expression in (1) Mouse heart tissue lysate; (2) Rat heart tissue lysate (M01948-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DES monoclonal antibody (Catalog # M01948-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DES
quantity: 100 µl
price: 315 USD
to the supplier
mouse monoclonal (2B5)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Phospho-Desmin (S60) Antibody
Boster
catalog: A01948S60
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
mouse monoclonal (DES/1711)
reactivity: human
application: immunohistochemistry
quantity: 100 ug/vial
price: 440 USD
to the supplier
domestic rabbit monoclonal (RM234)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 ul
price: 399 USD
to the supplier