Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband™
Boster
catalog: PB9124

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of ACE using anti-ACE antibody (PB9124). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, . Lane 2: mouse lung tissue lysates, . Lane 3: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # PB9124) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.

Figure 2. IHC analysis of ACE using anti-ACE antibody (PB9124). ACE was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of ACE using anti-ACE antibody (PB9124). ACE was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Angiotensin Converting Enzyme 1/ACE Antibody
Boster
catalog: PA2196-1

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti- ACE antibody, PA2196-1, IHC(P). IHC(P): Human Placenta Tissue

Anti- ACE antibody, PA2196-1, IHC(P). IHC(P): Human Prostatic Cancer Tissue

Anti- ACE antibody, PA2196-1, Western blotting. All lanes: Anti Angiotensin Converting Enzyme 1 (PA2196-1) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: 22RV1 Whole Cell Lysate at 40ug. Predicted bind size: 150KD. Observed bind size: 150KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Angiotensin Converting Enzyme 1/ACE Antibody
Boster
catalog: PA2196-2

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Anti- ACE antibody, PA2196-2, Western blotting. All lanes: Anti ACE (PA2196-2) at 0.5ug/ml. Lane 1: A549 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: 22RV1 Whole Cell Lysate at 40ug. Predicted bind size: 150KD. Observed bind size: 150KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Angiotensin-converting enzyme ACE Antibody
Boster
catalog: A00251-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-ACE1 Antibody
Boster
catalog: A00251-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-ACE1 Monoclonal Antibody
Boster
catalog: M00251

domestic rabbit monoclonal (ADCO-1)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

quantity: 100 µl
price: 315 USD
to the supplier

Anti-ACE1 Monoclonal Antibody
Boster
catalog: M00251-2

mouse monoclonal (monoclonal)
reactivity: human
application: western blot

quantity: 100 ul
price: 380 USD
to the supplier