Anti-IL-1 beta/IL1B Antibody Picoband™
Boster
catalog: A00101

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate,. Lane 2: rat spleen tissue lysate,. Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier

Anti-IL1 beta/IL1B Antibody
Boster
catalog: PA1351

domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot

Anti-IL-1 beta antibody, PA1351, Western blotting. Lane 1: Recombinant Mouse IL-1 beta Protein 10ng. Lane 2: Recombinant Mouse IL-1 beta Protein 5ng. Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IL1 beta/IL1B Antibody Picoband™
Boster
catalog: PB9025

domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, ELISA

Anti-IL1 beta Picoband antibody, PB9025-1.jpg. All lanes: Anti-IL-1 beta(PB9025) at 0.5ug/ml. WB: Rat Testis Tissue Lysate at 40ug. Predicted bind size: 31KD. Observed bind size: 35KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bid Il1b Antibody Picoband™
Boster
catalog: PB9027

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IL1 beta/Il1b Antibody Picoband™
Boster
catalog: A00101-1

domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot

Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 31KD. The expected band size for IL1 beta is at 31KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier

Anti-IL1 beta Antibody
Boster
catalog: A00101-3

domestic rabbit polyclonal (polyclonal)
reactivity: mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 ug
price: 487 USD
to the supplier

Anti-IL-1 beta Rabbit Monoclonal Antibody
Boster
catalog: M00101-3

domestic rabbit monoclonal (28I10)
reactivity: mouse
application: western blot, immunoprecipitation, flow cytometry

quantity: 100 µl/vial
price: 315 USD
to the supplier