Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody
Boster
catalog: M00393-1

domestic rabbit monoclonal (AOFD-9)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of Interferon gamma expression in Jurkat cell lysate (M00393-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG monoclonal antibody (Catalog # M00393-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Interferon gamma Ifng Antibody Picoband™
Boster
catalog: A00393-2

domestic rabbit polyclonal
reactivity: mouse
application: ELISA

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Interferon gamma/IFNG Antibody Picoband™
Boster
catalog: A00393-3

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human HL-60 whole cell lysates,. Lane 5: human U-937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 2. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat lung tissue lysates,. Lane 3: mouse brain tissue lysates,. Lane 4: mouse ovary tissue lysates,. Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 3. IHC analysis of IFNG using anti-IFNG antibody (A00393-3). IFNG was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Interferon gamma Biotinylated Ifng Antibody
Boster
catalog: A00393-1-Biotin

domestic rabbit polyclonal
reactivity: mouse
conjugate: biotin
application: ELISA

Boster Kit Box
quantity: 50µg/vial
price: 315 USD
to the supplier

Anti-IFN-Gamma (Q69) IFNG Antibody
Boster
catalog: A00393

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

quantity: 100 ul
price: 512 USD
to the supplier

Anti- IFN Gamma Monoclonal Antibody
Boster
catalog: M00393-5

rat monoclonal (AFII-9)
reactivity: mouse
application: immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 299 USD
to the supplier