mouse monoclonal (CTD-19)
reactivity: human, rat
application: western blot, immunohistochemistry
reactivity: human, rat
application: western blot, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
Anti-Cathepsin D Picoband antibody, PB9047-1.JPG. IHC(P): Human Lung Cancer Tissue
Anti-Cathepsin D Picoband antibody, PB9047-2.jpg. All lanes: Anti-Cathepsin D(PB9047) at 0.5ug/ml. WB: Recombinant Human Cathepsin D Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (PB9854). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates, . Lane 2: A549 whole cell lysates,. Lane 3: PANC1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # PB9854) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 28KD. The expected band size for Cathepsin D is at 28KD.
Figure 2. IHC analysis of Cathepsin D using anti- Cathepsin D antibody (PB9854). Cathepsin D was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cathepsin D Antibody (PB9854) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (CEF-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Immunohistochemical analysis on paraffin-embedded tisf paraffin-embedded human ovarian cancer tissue, using Cathepsin D Antibody(M01361). CTSD was detected isue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTSD Antibody (M01361)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Cathepsin D expression in (1)MCF-7 cell lysate;(2)SKBR-3 cell lysate (M01361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSD monoclonal antibody (Catalog # M01361) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTSD
quantity: 100 µl
price: 315 USD
to the supplier