domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Anti- Stefin B Picoband antibody, PB9565, Western blotting. All lanes: Anti Stefin B (PB9565) at 0.5ug/ml. Lane 1: Human Placenta Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A431 Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Predicted bind size: 11KD. Observed bind size: 11KD
Anti- Stefin B Picoband antibody, PB9565,IHC(P). IHC(P): Human Prostatic Cancer Tissue
Figure 3. Flow Cytometry analysis of A431 cells using anti- Stefin B antibody (PB9565). Overlay histogram showing A431 cells stained with Stefin B (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Stefin B Antibody (Stefin B,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (2B6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Figure 1. Western blot analysis of Stefin B using anti-Stefin B antibody (M02794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, . Lane 2: human SW620 whole cell lysates, . Lane 3: human U-937 whole cell lysates, . Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Stefin B antigen affinity purified monoclonal antibody (Catalog # M02794) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Figure 2. IHC analysis of Stefin B using anti-Stefin B antibody (M02794). Stefin B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Stefin B Antibody (M02794) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Human Stefin B DyLight® 488 conjugated CSTB Antibody(monoclonal, 2B6)
Boster
catalog: M02794-Dyl488
Boster
catalog: M02794-Dyl488
mouse monoclonal (2B6)
reactivity: human
conjugate: DyLight488
application: flow cytometry
reactivity: human
conjugate: DyLight488
application: flow cytometry
Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody(monoclonal, 2B6)
Boster
catalog: M02794-Dyl550
Boster
catalog: M02794-Dyl550
mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
