Anti-Cystatin C/CST3 Antibody Picoband™
Boster
catalog: A00961-2

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,. Lane 2: human placenta tissue lysates, . Lane 3: human HepG2 whole cell lysates,. Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.

Figure 2. IHC analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). Cystatin C was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cystatin C Antibody (A00961-2) overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Cystatin C/CST3 Antibody Picoband™ (monoclonal, 4H8)
Boster
catalog: M00961-1

mouse monoclonal (4H8)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)
Boster
catalog: M00961-Biotin

mouse monoclonal (8D1)
reactivity: human
application: ELISA

Boster Kit Box
quantity: 50µg/vial
price: 315 USD
to the supplier

Anti-Cystatin C/CST3 Antibody Picoband™ (monoclonal, 8H7)
Boster
catalog: M00961-3

mouse monoclonal (8H7)
reactivity: human
application: ELISA

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Cystatin C CST3 Rabbit Monoclonal Antibody
Boster
catalog: M00961

domestic rabbit monoclonal (IEE-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Cystatin C expression in HeLa cell lysate (M00961). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CST3 monoclonal antibody (Catalog # M00961) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CST3
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Cystatin C CST3 Antibody
Boster
catalog: A00961-4

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 697 USD
to the supplier