domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
reactivity: human, mouse
application: western blot, ELISA
domestic rabbit monoclonal (ABAC-3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of G-CSF expression in K562 cell lysate (M02280-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF3 monoclonal antibody (Catalog # M02280-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF3
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
reactivity: human
application: western blot
mouse monoclonal (SPM468)
reactivity: human
application: immunohistochemistry
reactivity: human
application: immunohistochemistry