Anti-PARP/PARP1 Antibody Picoband™
Boster
catalog: PB9309

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of PARP using anti-PARP antibody (PB9309). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human HepG2 whole cell lysates,. Lane 3: human COLO-320 whole cell lysates, . Lane 4: human Jurkat whole cell lysates,. Lane 5: rat PC-12 whole cell lysates,. Lane 6: mouse NIH3T3 whole cell lysates,. Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP antigen affinity purified polyclonal antibody (Catalog # PB9309) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP at approximately 120KD. The expected band size for PARP is at 113KD.

Figure 2. IHC analysis of PARP using anti-PARP antibody (PB9309). PARP was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of PARP using anti-PARP antibody (PB9309). PARP was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-PARP PARP1 Rabbit Monoclonal Antibody
Boster
catalog: M00122

domestic rabbit monoclonal (ABCG-16)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of PARP expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with staurosporine (M00122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
Boster
catalog: M00122-1

domestic rabbit monoclonal (HI-16)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Cleaved PARP expression in (1) RAW2647 cell lysate;(2) NIH/3T3 cell lysate (M00122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1

Immunohistochemical analysis of paraffin-embedded human kidney, using Cleaved PARP Antibody(M00122-1). PARP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARP1 Antibody (M00122-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
Boster
catalog: M00122-2

domestic rabbit monoclonal (BHH-16)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Cleaved PARP expression in Jurkat cell lysate (M00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-PARP PARP1 Rabbit Monoclonal Antibody
Boster
catalog: M00122-3

domestic rabbit monoclonal (CFD-16)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of PARP expression in Jurkat cell lysate (M00122-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122-3) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-PARP Picoband™ Antibody (monoclonal, 2I2H4)
Boster
catalog: M00122-4

mouse monoclonal (2I2H4)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-PARP Picoband™ Antibody (monoclonal, 10E11)
Boster
catalog: M00122-5

mouse monoclonal (110)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-PARP Picoband™ Antibody (monoclonal, 10G9)
Boster
catalog: M00122-6

mouse monoclonal (10G9)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier