Anti-H4K20me1 Antibody
Boster
catalog: CI1005

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H4K20me3 Antibody
Boster
catalog: CI1011

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H4K8ac Antibody
Boster
catalog: CI1021

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H4K5,8,12ac Antibody
Boster
catalog: CI1033

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/66 ul
price: 380 USD
to the supplier

Anti-H4K5,8,12,16ac Antibody
Boster
catalog: CI1035

rabbit polyclonal
reactivity: human
application: immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/66 ul
price: 380 USD
to the supplier

Anti-H4K5ac Antibody
Boster
catalog: CI1036

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/28 ul
price: 380 USD
to the supplier

Anti-H4K20me1 Antibody
Boster
catalog: CI1039

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 50 ug/25 ul
price: 380 USD
to the supplier

Anti-H4K20me3 Antibody
Boster
catalog: CI1050

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/47 ul
price: 380 USD
to the supplier

Anti-H4K8ac Antibody
Boster
catalog: CI1066

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/41 ul
price: 380 USD
to the supplier

Anti-H4pan Antibody
Boster
catalog: CI1069

rabbit polyclonal
reactivity: human
application: ELISA, chromatin immunoprecipitation

quantity: 50 ug/44 ul
price: 380 USD
to the supplier

Anti-Histone H4 (tri methyl K20) Rabbit Monoclonal Antibody
Boster
catalog: M14495

rabbit monoclonal (DHB-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Histone H4 (tri methyl K20) expression in HeLa cell lysate (M14495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody (Catalog # M14495) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H4A

Immunohistochemical analysis of paraffin-embedded human stomach, using Histone H4 (tri methyl K20) Antibody(M14495). HIST1H4A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal Antibody, Clone#RM194
Boster
catalog: P14495

rabbit monoclonal (RM194)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: 360 USD
to the supplier

Anti-Histone H4 (acetyl K16) Monoclonal Antibody
Boster
catalog: M14495-3

rabbit monoclonal (COC-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A antigen affinity purified polyclonal antibody (Catalog # M14495-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H4A.

Figure 2. IHC analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-3). HIST1H4A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495-3) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Immunofluorescent analysis of HeLa cells treated with TSA, using Histone H4 (acetyl K16) Antibody .
quantity: 100 ug
price: 299 USD
to the supplier

Anti-Histone H4 (acetyl K5) Monoclonal Antibody
Boster
catalog: M14495-4

rabbit monoclonal (CCA-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Figure 2. IHC analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-4). HIST1H4A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495-4) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of HIST1H4A using anti-HIST1H4A antibody (M14495-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A antigen affinity purified polyclonal antibody (Catalog # M14495-4) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H4A.
quantity: 100 ug
price: 299 USD
to the supplier

Anti-Histone H4 (mono methyl K16) Rabbit Monoclonal Antibody
Boster
catalog: M14495-1

rabbit monoclonal (DEF-8)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of Histone H4 (mono methyl K16) expression in (1) NIH/3T3 cell lysate; (2) A549 cell lysate (M14495-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody (Catalog # M14495-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H4A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H4 Rabbit Monoclonal Antibody
Boster
catalog: M14495-2

rabbit monoclonal (HDC-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

Western blot analysis of Histone H4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (M14495-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody (Catalog # M14495-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H4A
quantity: 100 ug/vial
price: 299 USD
to the supplier