Anti-Apolipoprotein J/CLU Antibody Picoband™
Boster
catalog: PB9575

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Anti- Apolipoprotein J Picoband antibody, PB9575, Western blotting. All lanes: Anti Apolipoprotein J (PB9575) at 0.5ug/ml. Lane 1: SKOV Whole Cell Lysate at 40ug. Lane 2: U87 Whole Cell Lysate at 40ug. Lane 3: PANC Whole Cell Lysate at 40ug. Predicted bind size: 52KD. Observed bind size: 82KD

Anti- Apolipoprotein J Picoband antibody, PB9575, IHC(P). IHC(P): Human Testis Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Clusterin Antibody
Boster
catalog: A00590

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-Clusterin-Alpha (E436) Antibody
Boster
catalog: A00590-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-CLU Antibody (N-term)
Boster
catalog: A00590-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

quantity: 80 ul
price: 166 USD
to the supplier

Anti-Clusterin (human) Monoclonal Antibody (CLI-9)
Boster
catalog: M00590

mouse monoclonal (CLI-9)
reactivity: human
application: immunocytochemistry, immunoprecipitation

quantity: 100µg
price: 384 USD
to the supplier

Anti-Clusterin Purified Monoclonal Antibody
Boster
catalog: M00590-1

mouse monoclonal (HS-3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 0.1 mg (C100)
price: 280 USD
to the supplier

Anti-Clusterin Rabbit Monoclonal Antibody
Boster
catalog: M00590-2

domestic rabbit monoclonal (17C92)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 µl/vial
price: 315 USD
to the supplier

Anti-Clusterin/CLU Antibody Picoband™
Boster
catalog: A00590-3

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clusterin/CLU antigen affinity purified polyclonal antibody (Catalog # A00590-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clusterin/CLU at approximately 70 kDa. The expected band size for Clusterin/CLU is at 52 kDa.

Figure 2. IHC analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Clusterin/CLU was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Clusterin/CLU Antibody (A00590-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
quantity: 100 µg/vial
price: 315 USD
to the supplier