domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-IKK alpha antibody, PA1614, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: JURKAT Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti-IKK alpha Picoband antibody, PB9110-1.jpg. All lanes: Anti IKKA (PB9110) at 0.5ug/ml. WB: Recombinant Human IKKA Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD
Anti-IKK alpha Picoband antibody, PB9110-2.jpg. All lanes: Anti IKKA (PB9110) at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: SGC Whole Cell Lysate at 40ug. Lane 3: PANC Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: Mouse Cardiac Muscle Tissue Lysate at 50ug. Predicted bind size: 85KD. Observed bind size: 85KD
Anti-IKK alpha Picoband antibody, PB9110-3.JPG. IHC(P): Rat Spleen Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (FCC-3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of IKK alpha+beta expression in A431 cell lysate (M01918). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHUK monoclonal antibody (Catalog # M01918) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHUK
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (GOD-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of IKK alpha expression in Daudi cell lysate (M01918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHUK monoclonal antibody (Catalog # M01918-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHUK
quantity: 100 µl
price: 315 USD
to the supplier