domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Figure 1. Western blot analysis of Chk2 using anti- Chk2 antibody (PA1202). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-231 whole cell lysates, . Lane 2: human MDA-MB-453 whole cell lysates, . Lane 3: human HepG2 whole cell lysates, . Lane 4: human K562 whole cell lysates,. Lane 5: human Caco-2 whole cell lysates,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Chk2 antigen affinity purified polyclonal antibody (Catalog # PA1202) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk2 at approximately 69KD. The expected band size for Chk2 is at 61KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti- Chk2 Picoband antibody, PB9692, Western blotting. All lanes: Anti Chk2 (PB9692) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: Mouse Testis Tissue Lysate at 50ug. Lane 3: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 65KD. Observed bind size: 65KD
Anti- Chk2 Picoband antibody, PB9692, IHC(P). IHC(P): Human Lung Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (FOF-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate (M00277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 monoclonal antibody (Catalog # M00277) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (27C74)
reactivity: human
application: western blot, immunoprecipitation
reactivity: human
application: western blot, immunoprecipitation