domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of Chk1 using anti- Chk1 antibody (A01060). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Chk1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1 at approximately 54KD. The expected band size for Chk1 is at 54KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AAF-3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate (M01060-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # M01060-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (EIB-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
Western blot analysis of Chk1 expression in HeLa cell lysate (M01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # M01060) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (FEO-3)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin (P01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody (Catalog # P01060) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK1
quantity: 100 µl
price: 315 USD
to the supplier