domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-Podoplanin/gp36 antibody, PA1411, Western blotting. All lanes: Anti Podoplanin/gp36 (PA1411) at 0.5ug/ml. Lane 1: SMMC Whole Cell Lysate at 40ug. Lane 2: 293T Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Predicted bind size: 36KD. Observed bind size: 36KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-Podoplanin/gp36 antibody, PA1674, Western blotting. All lanes: Anti PDPN (PA1674) at 0.5ug/ml. Lane 1: A549 Whole Cell Lysate at 40ug. Lane 2: SMMC Whole Cell Lysate at 40ug. Lane 3: 293T Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 36KD. Observed bind size: 36KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AACC-16)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Podoplanin expression in human placenta lysate (M01124-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPN monoclonal antibody (Catalog # M01124-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPN
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (AAHB-16)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
Western blot analysis of Podoplanin expression in 293T cell lysate (M01124-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPN monoclonal antibody (Catalog # M01124-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPN
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry
reactivity: human
application: western blot, ELISA, flow cytometry