Anti-CEACAM5/Cd66e Monoclonal Antibody
Boster
catalog: MA1023

mouse monoclonal (C6G9)
reactivity: human
application: western blot, immunohistochemistry

Figure 1. Western blot analysis of CEACAM5/Cd66e using anti- CEACAM5/Cd66e antibody (MA1023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Recombinant Human CEACAM5/Cd66e Protein 10ng. Lane 2; Recombinant Human CEACAM5/Cd66e Protein 5ng. Lane 3: Recombinant Human CEACAM5/Cd66e Protein 2.5ng. Lane 4: Recombinant Human CEACAM5/Cd66e Protein 1.25ng . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CEACAM5/Cd66e antigen affinity purified monoclonal antibody (Catalog # MA1023) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Figure 2. IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). CEACAM5/Cd66e was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CEACAM5/Cd66e Antibody (MA1023) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CEACAM5 Antibody
Boster
catalog: RP1018

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of CEA using anti- CEA antibody (RP1018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, . Lane 2: human Caco-2 whole cell lysates, . Lane 3: mouse small intestine tissue lysates, . Lane 4: mouse stomach tissue lysates,. Lane 5: mouse lung tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse NIH3T3 whole cell lysates,. Lane 8: mouse HEPA1-6 whole cell lysates,. Lane 9: mouse SP20 whole cell lysates,. Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CEACAM5 Antibody Picoband™
Boster
catalog: A00356

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CEA Antibody (Center)
Boster
catalog: A00356-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

quantity: 80 ul
price: 166 USD
to the supplier

Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody
Boster
catalog: M00356-1

domestic rabbit monoclonal (AAOA-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 µl
price: 315 USD
to the supplier

Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326
Boster
catalog: M00356-3

domestic rabbit monoclonal (RM326)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier