Anti-Cytochrome C/Cycs Picoband Antibody
Boster
catalog: A03529

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Figure 5. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529). . Cytochrome C was detected in paraffin-embedded section of rat intestine tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cytochrome C Antibody (A03529) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529). . Cytochrome C was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cytochrome C Antibody (A03529) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529). . Cytochrome C was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cytochrome C Antibody (A03529) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier

Anti-Cytochrome C Antibody
Boster
catalog: PA1118

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 ug/vial
price: 200 USD
to the supplier

Anti-Cytochrome C/CYCS Picoband Antibody
Boster
catalog: PB9334

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,. Lane 2: Mouse Brain Tissue Lysate,. Lane 3: Rat Cardiac Muscle Tissue Lysate,. Lane 4: Mouse Cardiac Muscle Tissue Lysate,. Lane 5: U87 Whole Cell Lysate,. Lane 6: NEURO Whole Cell Lysate,. Lane 7: HELA Whole Cell Lysate,. Lane 8: JURKAT Whole Cell Lysate,. Lane 9: Human Placenta Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody (Catalog # PB9334) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 14KD.

Figure 2. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). Cytochrome C was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). Cytochrome C was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier

Anti-Cytochrome C Picoband Antibody (monoclonal, 15F10)
Boster
catalog: M03529-5

mouse monoclonal (15F10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5). Overlay histogram showing A431 cells stained with M03529-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 2. Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5). Overlay histogram showing A431 cells stained with M03529-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100 ug/vial
price: 280 USD
to the supplier

Anti-Cytochrome C Rabbit Monoclonal Antibody
Boster
catalog: M03529-1

rabbit monoclonal (FOA-3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Cytochrome C expression in HeLa cell lysate (M03529-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody (Catalog # M03529-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Cytochrome C Rabbit Monoclonal Antibody
Boster
catalog: M03529-2

rabbit monoclonal (GOC-3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Cytochrome C expression in Human kidney lysate (M03529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody (Catalog # M03529-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS
quantity: 100 ug/vial
price: 299 USD
to the supplier