rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat, zebrafish
application: western blot, ELISA, immunohistochemistry
Figure 1. Western blot analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, . Lane 2: mouse testis tissue lysates,. Lane 3: rat spleen tissue lysates,. Lane 4: human A549 whole cell lysates,. Lane 5: human placenta tissue lysates,. Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3 / GST pi antigen affinity purified polyclonal antibody (Catalog # A00394) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3 / GST pi at approximately 23KD. The expected band size for GST3 / GST pi is at 23KD.
Figure 2. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394). GST3 / GST pi was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394). GST3 / GST pi was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Anti-GST3/GST pi antibody, PA1040, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: COLO320 Cell Lysate.
quantity: 100 ug/vial
price: 240 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti-GST3/GST pi antibody, PA1590, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Anti-GST3/GST pi antibody, PA1590,Western blotting. All lanes: Anti GST3/GST pi (PA1590) at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 23KD. Observed bind size: 23KD
quantity: 100 ug/vial
price: 280 USD
to the supplier
rabbit monoclonal (DD-7)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of GST expression in GST recombinant protein (M00394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTP1 monoclonal antibody (Catalog # M00394) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTP1
quantity: 100 ug/vial
price: 299 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat, zebrafish
application: western blot, ELISA, immunohistochemistry
Figure 1. Western blot analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, . Lane 2: rat liver tissue lysates,. Lane 3: mouse lung tissue lysates,. Lane 4: mouse liver tissue lysates,. Lane 5: mouse spleen tissue lysates,. Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3 / GST pi antigen affinity purified polyclonal antibody (Catalog # A00394-1) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3 / GST pi at approximately 23KD. The expected band size for GST3 / GST pi is at 23KD.
Figure 2. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394-1). GST3 / GST pi was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of GST3 / GST pi using anti-GST3 / GST pi antibody (A00394-1). GST3 / GST pi was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GST3 / GST pi Antibody (A00394-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 280 USD
to the supplier