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> Boster N-cadherin antibody
Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody
Boster
catalog: MA1067
mouse monoclonal (GC-4)
reactivity:
human
,
mouse
,
rat
,
chicken
application:
western blot
,
immunohistochemistry
Figure 1. Western blot analysis of Cadherin-2/N-Cadherin using anti- Cadherin-2/N-Cadherin antibody (MA1067). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human A431 whole cell lysates,. Lane 3: human MCF-7 whole cell lysates, . Lane 4: human PANC-1 whole cell lysates,. Lane 5: human A549 whole cell lysates,. Lane 6: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Cadherin-2/N-Cadherin antigen affinity purified monoclonal antibody (Catalog # MA1067) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cadherin-2/N-Cadherin at approximately 130KD. The expected band size for Cadherin-2/N-Cadherin is at 100KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-N-Cadherin-2 CDH2 CD325-Antibody
Boster
catalog: PA1328
domestic rabbit polyclonal
reactivity:
human
,
mouse
,
rat
application:
western blot
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
Figure 1. Western blot analysis of N Cadherin using anti-N Cadherin antibody (PA1328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N Cadherin antigen affinity purified polyclonal antibody (Catalog # PA1328) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N Cadherin at approximately 140KD. The expected band size for N Cadherin is at 100KD.
Anti-N Cadherin antibody, PA1328, IHC(P). IHC(P): Human Ovary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody
Boster
catalog: M01577-1
domestic rabbit monoclonal (FI-3)
reactivity:
human
,
mouse
,
rat
application:
western blot
,
immunohistochemistry
Western blot analysis of N-Cadherin expression in HeLa cell lysate (M01577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 monoclonal antibody (Catalog # M01577-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2
quantity: 100 µl
price: 315 USD
to the supplier
Anti-N-Cadherin-2 CDH2 CD325-Antibody
Boster
catalog: A01577-1
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
quantity: 100 ul
price: 697 USD
to the supplier
Anti-N-Cadherin-2 CDH2 CD325-Antibody Picoband™
Boster
catalog: A01577-2
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-N Cadherin/CDH2 Antibody Picoband™
Boster
catalog: A01577-3
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
ELISA
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody, Clone#RM259
Boster
catalog: M01577-2
domestic rabbit monoclonal (RM259)
reactivity:
human
application:
western blot
quantity: 100 uL
price: 399 USD
to the supplier