Boster Immunoleader

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Western Blotting Rat IgG DAB Chromogenic Kit (Yellow)

SA2023

1 kit

90 USD

Western Blotting Rat IgG DAB Chromogenic Kit (Yellow)

SA2023

90 USD

1 kit

‐20˚C for 1 year. DAB reagent should be protected from light.

WB

1. Blocking Reagent: 2x10g protein dry powder. 2. Concentrated antibody diluent solution: 20 ml/bottle. 10x. 3. Polymeric HRP‐conjugated rabbit anti‐rat IgG: 0.2 ml. 200‐400x. 4. DAB chromogenic reagent, containing: • Chromogenic reagent A: DAB concentrated solution, 3ml. 40x. • Chromogenic reagent B: H2O2 concentrated solution, 3ml. 40x. • Chromogenic reagent C: TBS concentrated buffer, 3ml. 40x.

•Nitrocellulose or PVDF membrane. •Diluent Buffer (for preparation of blocking reagent and antibody dilutions): Add 2.42g Tris, 9g NaCl, 850‐900ul pure acetic acid into 1000ml distilled water, adjust pH to 7.2‐7.6. •Wash Buffer: Add 0.5 ml of TWEEN 20 into 1000 ml of diluent buffer. •Primary antibody: User provided. This kit applies to the primary antibodies raised from rat.

• “Rat IgG” refers to the origin of animal species of the primary antibody, not the origin of the specimen. This kit applies to the primary antibodies raised from rat. • Store the kit at 4˚C for frequent use, or at ‐20˚C for infrequent use. The shelf life at ‐20˚C is one year. • Chromogenic reagent A should be stored at ‐20˚C. If crystal appears, fully dissolve it before use. • Chromogenic working solution should be freshly prepared.