Anti-DMRT1 Antibody | Boster Immunoleader RP1083 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-DMRT1 Antibody

catalog :

RP1083

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-DMRT1 Antibody

Catalog Number :

RP1083

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Doublesex- and mab-3-related transcription factor 1(DMRT1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1 (98-128aa RDCQCKKCNLIAERQRVMAAQVALRRQQAQE), different from the related mouse sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Reference :

1. "Entrez Gene: DMRT1 doublesex and mab-3 related transcription factor 1". 2. Raymond CS, Shamu CE, Shen MM, Seifert KJ, Hirsch B, Hodgkin J, Zarkower D (February 1998). "Evidence for evolutionary conservation of sex-determining genes".Nature 391 (6668): 691–5. 3. Smith CA, Roeszler KN, Ohnesorg T, Cummins DM, Farlie PG, Doran TJ, Sinclair AH (August 2009). "The avian Z-linked gene DMRT1 is required for male sex determination in the chicken". Nature 461(7261): 267–71.

Gene Name :

DMRT1

Protein Name :

Doublesex- and mab-3-related transcription factor 1

Gene Full Name :

doublesex and mab-3 related transcription factor 1

Synonyms :

CT154 antibody DM domain expressed in testis 1 antibody DM domain expressed in testis protein 1 antibody DM domain gene expressed in testis antibody DMRT 1 antibody dmrt1 antibody DMRT1_HUMAN antibody DMT 1 antibody DMT1 antibody Doublesex and mab 3 related transcription factor 1 antibody Doublesex- and mab-3-related transcription factor 1 antibody

Uniprot ID :

Q9Y5R6

more info or order :

Boster Immunoleader product webpage