Anti-CYP7A1 Antibody | Boster Immunoleader RP1079 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CYP7A1 Antibody

catalog :

RP1079

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CYP7A1 Antibody

Catalog Number :

RP1079

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cholesterol 7-alpha-monooxygenase(CYP7A1) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA -HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.

Reference :

1. Agellon, L. B., Drover, V. A. B., Cheema, S. K., Gbaguidi, G. F., Walsh, A. Dietary cholesterol fails to stimulate the human cholesterol 7-alpha-hydroxylase gene (CYP7A1) in transgenic mice. J. Biol. Chem. 277: 20131-20134, 2002. 2. Cohen, J. C., Cali, J. J., Jelinek, D. F., Mehrabian, M., Sparkes, R. S., Lusis, A. J., Russell, D. W., Hobbs, H. H. Cloning of the human cholesterol 7-alpha-hydroxylase gene (CYP7) and localization to chromosome 8q11-q12. Genomics 14: 153-161, 1992. 3. Molowa, D. T., Chen, W. S., Cimis, G. M., Tan, C. P. Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene. Biochemistry 31: 2539-2544, 1992.

Gene Name :

CYP7A1

Protein Name :

Cholesterol 7-alpha-monooxygenase

Gene Full Name :

cytochrome P450, family 7, subfamily A, polypeptide 1

Synonyms :

Cholesterol 7 alpha hydroxylase antibody Cholesterol 7 alpha monooxygenase antibody cholesterol 7 alpha-monooxygenase antibody Cholesterol 7-alpha-hydroxylase antibody Cholesterol 7-alpha-monooxygenase antibody CP7A antibody CP7A1_HUMAN antibody CYP 7 antibody CYP7 antibody CYP7A1 antibody CYPVII antibody Cytochrome P450 7A1 antibody cytochrome P450, family 7, subfamily A, polypeptide 1 antibody Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), antibody Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody MGC126826 antibody MGC138389 antibody

Uniprot ID :

P22680

more info or order :

Boster Immunoleader product webpage