Anti-MAFA Antibody | Boster Immunoleader RP1066 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MAFA Antibody

catalog :

RP1066

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Availability :

Frp1066

Product Name :

Anti-MAFA Antibody

Catalog Number :

RP1066

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcription factor MafA(MAFA) detection. Tested with WB in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Notes :

WB: The detection limit for MAFA is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human MAFA(136-167aa EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR), identical to the related mouse sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.

Reference :

1. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002. 2. Zhang, C., Moriguchi, T., Kajihara, M., Esaki, R., Harada, A., Shimohata, H., Oishi, H., Hamada, M., Morito, N., Hasegawa, K., Kudo, T., Engel, J. D., Yamamoto, M., Takahashi, S. MafA is a key regulator of glucose-stimulated insulin secretion. Molec. Cell. Biol. 25: 4969-4976, 2005.

Gene Name :

MAFA

Protein Name :

Transcription factor MafA

Gene Full Name :

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A

Synonyms :

HMafA antibody Mafa antibody MAFA_HUMAN antibody Pancreatic beta cell specific transcriptional activator antibody Pancreatic beta-cell-specific transcriptional activator antibody RIPE3b1 antibody Transcription factor MafA antibody Transcription factor RIPE3b1 antibody v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody

Uniprot ID :

Q8NHW3

more info or order :

Boster Immunoleader product webpage