Anti-HGF Antibody | Boster Immunoleader RP1062 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HGF Antibody

catalog :

RP1062

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse

application :

western blot

publications citing this reagent :

Lin Y, Xie W, Chen Y, Zhang X, Zeng X, Qiang H, et al. Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes. Liver Int. 2005;25:796-807 pubmed

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Boster Immunoleader product webpage

product information

Product Name :

Anti-HGF Antibody

Catalog Number :

RP1062

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Hepatocyte growth factor(HGF) detection. Tested with WB in Mouse.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Notes :

WB: The detection limit for HGF is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64aa QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. This gene is mapped to 7q21.11. It is secreted by mesenchymal cells and targets and acts primarily uponepithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration and in wound healing. HGF can regulate cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF also serves as a paracrine mediator to control placental development and growth. HGF was identified as one of the liver sinusoidal endothelial cell-derived paracrine mediators promoting hepatocyte growth.

Reference :

1. Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA (February 1991). "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product". Science251 (4995): 802–4. 2. Gallagher, J.T., Lyon, M. (2000). "Molecular structure of Heparan Sulfate and interactions with growth factors and morphogens". In Iozzo, M, V. Proteoglycans: structure, biology and molecular interactions. Marcel Dekker Inc. New York, New York. pp. 27–59. 3. LeCouter, J., Moritz, D. R., Li, B., Phillips, G. L., Liang, X. H., Gerber, H.-P., Hillan, K. J., Ferrara, N. Angiogenesis-independent endothelial protection of liver: role of VEGFR-1. Science 299: 890-893, 2003.

Gene Name :

HGF

Protein Name :

Hepatocyte growth factor

Gene Full Name :

hepatocyte growth factor (hepapoietin A; scatter factor)

Synonyms :

DFNB39 antibody F TCF antibody Fibroblast derived tumor cytotoxic factor antibody Hepatocyte growth factor (hepapoietin A; scatter factor) antibody Hepatocyte growth factor antibody Hepatocyte growth factor beta chain antibody Hepatocyte growth factor precursor antibody Hepatopoeitin-A antibody Hepatopoietin A antibody Hgf antibody HGF_HUMAN antibody HGFB antibody HPTA antibody Lung fibroblast derived mitogen antibody OTTHUMP00000161349 antibody OTTHUMP00000206710 antibody OTTHUMP00000206711 antibody OTTHUMP00000206712 antibody OTTHUMP00000206713 antibody OTTHUMP00000206730 antibody Scatter factor antibody SF antibody

Uniprot ID :

Q08048

more info or order :

Boster Immunoleader product webpage