Boster Immunoleader

antibody

Anti-Midkine Antibody

RP1051

100 ug/vial

200 USD

polyclonal

rabbit

Anti-Midkine Antibody

RP1051

Polyclonal

Rabbit IgG polyclonal antibody for Midkine(MDK) detection. Tested with WB in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human

WB: The detection limit for Midkine is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Midkine recombinant protein (Position: V21-D143). Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Midkine(MK or MDK), also known as neurite growth-promoting factor 2(NEGF2), is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPζ), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.

1. Ezquerra, L., Herradon, G., Nguyen, T., Silos-Santiago, I., Deuel, T. F.Midkine, a newly discovered regulator of the renin-angiotensin pathway in mouse aorta: significance of the pleiotrophin/midkine developmental gene family in angiotensin II signaling.Biochem. Biophys. Res. Commun. 333: 636-643, 2005. 2. Inoh, K., Muramatsu, H., Ochiai, K., Torii, S., Muramatsu, T.Midkine, a heparin-binding cytokine, plays key roles in intraperitoneal adhesions.Biochem. Biophys. Res. Commun. 317: 108-113, 2004.

MDK

Midkine

midkine (neurite growth-promoting factor 2)

Amphiregulin associated protein antibody Amphiregulin-associated protein antibody ARAP antibody FLJ27379 antibody MDK antibody Midgestation and kidney protein antibody Midkine antibody MK 1 antibody MK antibody MK_HUMAN antibody MK1 antibody NEGF 2 antibody NEGF2 antibody neurite growth promoting factor 2 antibody Neurite outgrowth promoting protein antibody Neurite outgrowth-promoting factor 2 antibody Neurite outgrowth-promoting protein antibody Retinoic acid inducible factor antibody

P21741