Anti-MBD2 Antibody | Boster Immunoleader RP1049 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MBD2 Antibody

catalog :

RP1049

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MBD2 Antibody

Catalog Number :

RP1049

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 2(MBD2) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for MBD2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Reference :

1. Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A (September 1999). "Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes". Mamm Genome 10 (9): 906–12. 2. Hendrich, B., Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Molec. Cell. Biol. 18: 6538-6547, 1998.

Gene Name :

MBD2

Protein Name :

Methyl-CpG-binding domain protein 2

Gene Full Name :

methyl-CpG binding domain protein 2

Synonyms :

DEMETHYLASE antibody DKFZp586O0821 antibody DMTase antibody MBD 2 antibody Mbd2 antibody MBD2_HUMAN antibody MBD2a antibody Methyl CpG binding domain protein 2 antibody Methyl CpG binding protein MBD2 antibody Methyl-CpG-binding domain protein 2 antibody Methyl-CpG-binding protein MBD2 antibody NY CO 41 antibody

Uniprot ID :

Q9UBB5

more info or order :

Boster Immunoleader product webpage