Anti-Dicer Antibody | Boster Immunoleader RP1044 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Dicer Antibody

catalog :

RP1044

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Dicer Antibody

Catalog Number :

RP1044

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Dicer is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Reference :

1. Hutvagner, G., McLachlan, J., Pasquinelli, A. E., Balint, E., Tuschl, T., Zamore, P. D. A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838, 2001. 2. Matsuda, S., Ichigotani, Y., Okuda, T., Irimura, T., Nakatsugawa, S., Hamaguchi, M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta 1490: 163-169, 2000.

Gene Name :

DICER1

Protein Name :

Endoribonuclease Dicer

Gene Full Name :

dicer 1, ribonuclease type III

Synonyms :

DCR antibody DCR1 antibody Dcr1 homolog (Drosophila) antibody Dicer 1 antibody Dicer 1 ribonuclease type III antibody Dicer antibody DICER_HUMAN antibody Dicer1 antibody Double-strand-specific ribonuclease antibody Endoribonuclease Dicer antibody Helicase moi antibody Helicase with RNAse motif antibody HERNA antibody KIAA0928 antibody MNG1 antibody RMSE2 antibody

Uniprot ID :

Q9UPY3

more info or order :

Boster Immunoleader product webpage