Anti-PD1 Antibody | Boster Immunoleader RP1039 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PD1 Antibody

catalog :

RP1039

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

publications citing this reagent :

Wang Y, Cao Y, Yang X, Niu H, Sun L, Wang X, et al. Effect of TLR4 and B7-H1 on immune escape of urothelial bladder cancer and its clinical significance. Asian Pac J Cancer Prev. 2014;15:1321-6 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-PD1 Antibody

Catalog Number :

RP1039

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Programmed cell death protein 1(PDCD1) detection. Tested with WB in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Notes :

WB: The detection limit for PD1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human PD1 recombinant protein (Position: P101-L288). Human PD1 shares 59% amino acid (aa) sequence identity with mouse PD1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

PDCD1(Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. This gene is mapped to 2q37.3. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. The PDCD1 gene contains 5 exons. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. Using flow cytometric analysis, It has been found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART)-treated HIV-positive patients. PDCD1 upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines.

Reference :

1. Barber, D. L., Wherry, E. J., Masopust, D., Zhu, B., Allison, J. P., Sharpe, A. H., Freeman, G. J., Ahmed, R. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature 439: 682-687, 2006. 2. Dai, H., Wan, N., Zhang, S., Moore, Y., Wan, F., Dai, Z. Cutting edge: programmed death-1 defines CD8+CD122+ T cells as regulatory versus memory T cells. J. Immun. 185: 803-807, 2010. 3. Finger, L. R., Pu, J., Wasserman, R., Vibhakar, R., Louie, E., Hardy, R. R., Burrows, P. D., Billips, L. G. The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors. Gene 197: 177-187, 1997.

Gene Name :

PDCD1

Protein Name :

Programmed cell death protein 1

Gene Full Name :

programmed cell death 1

Synonyms :

CD279 antibody CD279 antigen antibody hPD l antibody hPD-1 antibody hSLE1 antibody PD 1 antibody PD-1 antibody PD1 antibody PDCD 1 antibody PDCD1 antibody PDCD1_HUMAN antibody Programmed cell death 1 antibody Programmed cell death protein 1 antibody Protein PD 1 antibody Protein PD-1 antibody SLEB2 antibody Systemic lupus erythematosus susceptibility 2 antibody

Uniprot ID :

Q15116

more info or order :

Boster Immunoleader product webpage