Anti-Collagen III Antibody | Boster Immunoleader RP1033 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Collagen III Antibody

catalog :

RP1033

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

publications citing this reagent :

Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, et al. CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages. Prostaglandins Other Lipid Mediat. 2015;116:64-75 pubmed publisher
Lin Y, Xie W, Chen Y, Zhang X, Zeng X, Qiang H, et al. Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes. Liver Int. 2005;25:796-807 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Collagen III Antibody

Catalog Number :

RP1033

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Collagen alpha-1(III) chain(COL3A1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Collagen III is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12

Reference :

1. Janeczko RA, Ramirez F (Oct 1989). "Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen". Nucleic Acids Res 17 (16): 6742. 2. Superti-Furga A, Gugler E, Gitzelmann R, Steinmann B (Jun 1988). "Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen". J Biol Chem 263 (13): 6226–32.

Gene Name :

COL3A1

Protein Name :

Collagen alpha-1(III) chain

Gene Full Name :

collagen, type III, alpha 1

Synonyms :

CO3A1_HUMAN antibody COL 3A1 antibody COL3A1 antibody Collagen alpha 1(III) chain antibody Collagen alpha-1(III) chain antibody Collagen III alpha 1 chain precursor antibody Collagen III alpha 1 polypeptide antibody Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody Collagen type III alpha 1 antibody Collagen type III alpha antibody EDS4A antibody Ehlers Danlos syndrome type IV, autosomal dominant antibody Fetal collagen antibody Type III collagen antibody

Uniprot ID :

P02461

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