Anti-ALOX5 Antibody | Boster Immunoleader RP1031 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ALOX5 Antibody

catalog :

RP1031

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ALOX5 Antibody

Catalog Number :

RP1031

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for ALOX5 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Reference :

1. Luo, M., Jones, S. M., Peters-Golden, M., Brock, T. G. Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B(4) synthetic capacity. Proc. Nat. Acad. Sci. 100: 12165-12170, 2003. 2. Chu, J., Pratico, D. 5-lipoxygenase as an endogenous modulator of amyloid-beta formation in vivo. Ann. Neurol. 69: 34-46, 2011.

Gene Name :

ALOX5

Protein Name :

Arachidonate 5-lipoxygenase

Gene Full Name :

arachidonate 5-lipoxygenase

Synonyms :

5 LO antibody 5 LOX antibody 5-lipoxygenase antibody 5-LO antibody 5-LOX antibody 5LOX antibody 5LPG antibody ALOX 5 antibody Alox5 antibody Arachidonate 5 lipoxygenase antibody Arachidonate 5-lipoxygenase antibody arachidonic 5-lipoxygenase alpha-10 isoform antibody arachidonic 5-lipoxygenase delta-10-13 isoform antibody arachidonic 5-lipoxygenase delta-13 isoform antibody arachidonic 5-lipoxygenase delta-p10 isoform antibody Arachidonic acid 5 lipoxygenase antibody Leukotriene A4 synthase antibody LOG 5 antibody LOG5 antibody LOX5_HUMAN antibody MGC163204 antibody

Uniprot ID :

P09917

more info or order :

Boster Immunoleader product webpage