Anti-VR1 Antibody | Boster Immunoleader RP1030 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-VR1 Antibody

catalog :

RP1030

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-VR1 Antibody

Catalog Number :

RP1030

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily V member 1(TRPV1) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for VR1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human VR1 recombinant protein (Position: F473-K839). Human VR1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat VR1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

The transient receptor potential cation channel, subfamily V, member 1 (TRPV1), also known as the capsaicin receptor is a protein which in humans is encoded by the TRPV1 gene. TRPV1 (also called Vanilloid receptor type 1) is a ligand-gated nonselective cation channel that is considered to be an important integrator of various pain stimuli such as endogenous lipids, capsaicin, heat, and low pH. In addition to expression in primary afferents, TRPV1 is also expressed in the CNS. Cui M et al. (2006) demonstrate that TRPV1 receptors in the CNS play an important role in pain mediated by central sensitization. And the significant CNS penetration is necessary for a TRPV1 antagonist to produce broad-spectrum analgesia. And TRPV1 also participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.

Reference :

1. Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (October 1997). "The capsaicin receptor: a heat-activated ion channel in the pain pathway". Nature 389 (6653): 816–24. doi:10.1038/39807. PMID 9349813. 2. Xue Q, Yu Y, Trilk SL, Jong BE, Schumacher MA (August 2001). "The genomic organization of the gene encoding the vanilloid receptor: evidence for multiple splice variants". Genomics 76 (1-3): 14–20. doi:10.1006/geno.2001.6582. PMID 11549313. 3. Cui M, Honore P, Zhong C, Gauvin D, Mikusa J, Hernandez G, Chandran P, Gomtsyan A, Brown B, Bayburt EK, Marsh K, Bianchi B, McDonald H, Niforatos W, Neelands TR, Moreland RB, Decker MW, Lee CH, Sullivan JP, Faltynek CR (2006). "TRPV1 receptors in the CNS play a key role in broad-spectrum analgesia of TRPV1 antagonists". J. Neurosci. 26 (37): 9385–93. doi:10.1523/JNEUROSCI.1246-06.2006. PMID 16971522. 4. Birder, L. A.; Nakamura, Y.; Kiss, S.; Nealen, M. L.; Barrick, S.; Kanai, A. J.; Wang, E.; Ruiz, G.; de Groat, W. C.; Apodaca, G.; Watkins, S.; Caterina, M. J. : Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1. Nature Neurosci. 5: 856-860, 2002.

Gene Name :

TRPV1

Protein Name :

Transient receptor potential cation channel subfamily V member 1

Gene Full Name :

transient receptor potential cation channel, subfamily V, member 1

Synonyms :

capsaicin receptor antibody DKFZp434K0220 antibody osm 9 like TRP channel 1 antibody Osm-9-like TRP channel 1 antibody OTRPC1 antibody Transient receptor potential cation channel subfamily V member 1 antibody TRPV 1 antibody TRPV1 antibody TRPV1_HUMAN antibody Vanilloid receptor 1 antibody Vanilloid receptor subtype 1 antibody VR 1 antibody VR1 antibody

Uniprot ID :

Q8NER1

more info or order :

Boster Immunoleader product webpage