Anti-human Angiostatin K1-3 Antibody | Boster Immunoleader RP1027 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-human Angiostatin K1-3 Antibody

catalog :

RP1027

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-human Angiostatin K1-3 Antibody

Catalog Number :

RP1027

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Plasminogen(PLG) detection. Tested with WB, IHC-P, ELISA in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,ELISA

Western blot :

Concentration: 0.1-0.5μg/ml; Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Species: Human

ELISA :

Concentration: 0.1-0.5μg/ml; Species: Human

Notes :

WB: The detection limit for PLG is approximately 0.5ng/lane under reducing conditions. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E. coli-derived human Angiostatin K1-3 recombinant protein(Position: C103-C352).

Contents :

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED50=70nM) relative to kringles 1-4(ED50 = 135nM).

Reference :

1. O’Relly MS, Holmgren L, Shin Y, Chen C, Rosenthal RA, Moses M et al. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 1994; 79: 315–328 2. Cao Y, Ji RW, Davidson D, Schaller J, Marti D, Sohndel S et al. Kringle domains of human angiostatin. JBC 1996; 271: 29461–29467

Gene Name :

PLG

Protein Name :

Plasminogen

Gene Full Name :

plasminogen

Synonyms :

Angiostatin antibody DKFZp779M0222 antibody Plasmin antibody Plasmin heavy chain A antibody Plasminogen antibody PLG antibody

Uniprot ID :

P00747

more info or order :

Boster Immunoleader product webpage