Anti-rat IL10 Antibody | Boster Immunoleader RP1016 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-rat IL10 Antibody

catalog :

RP1016

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat

application :

western blot, ELISA, immunohistochemistry - paraffin section

publications citing this reagent :

Wang T, Zhou Y, Chen X, Zhu A. Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression. Braz J Med Biol Res. 2014;47:738-45 pubmed

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Boster Immunoleader product webpage

product information

Product Name :

Anti-rat IL10 Antibody

Catalog Number :

RP1016

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Interleukin-10(IL10) detection. Tested with WB, IHC-P, ELISA in Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat

Applications :

WB,IHC-P,ELISA

Western blot :

Concentration: 0.1-0.5μg/ml; Species: Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Species: Rat

ELISA :

Concentration: 0.1-0.5μg/ml; Species: Rat

Notes :

By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E. coli-derived rat IL-10 recombinant protein(Position: S19-N178).

Contents :

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Interleukin-10(IL-10 or IL10), also known as human cytokine synthesis inhibitory factor(CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Reference :

1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".

Gene Name :

IL10

Protein Name :

Interleukin-10(IL-10)

Gene Full Name :

interleukin 10

Synonyms :

CSIF antibody Cytokine Synthesis Inhibitory Factor antibody GVHDS antibody IL 10 antibody IL-10 antibody IL10 antibody IL10A antibody Interleukin 10 antibody Interleukin-10 antibody Interleukin10 antibody MGC126450 antibody MGC126451 antibody T-cell growth inhibitory factor antibody TGIF antibody

Uniprot ID :

P29456

more info or order :

Boster Immunoleader product webpage