product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-human IL3 Antibody
catalog :
RP1008
quantity :
100 ug/vial
price :
240 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section
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product information
Product Name :
Anti-human IL3 Antibody
Catalog Number :
RP1008
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Human.
Price :
240 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human
Applications :
WB,IHC-P,ELISA,Neu,IP
Western blot :
Concentration: 0.1-0.5μg/ml; Species: Human
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-1μg/ml; Species: Human
ELISA :
Concentration: 0.1-0.5μg/ml; Species: Human
Neutralization :
Species: Human
Immunoprecipitation :
Species: Human
Notes :
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen :
E. coli-derived human IL-3 recombinant protein(Position: A20-F152).
Contents :
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Background :
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
Reference :
1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.
2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986.
3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984.
4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987.
5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.
Gene Name :
IL3
Protein Name :
Interleukin-3(IL-3)
Gene Full Name :
interleukin 3(colony-stimulating factor, multiple)
Synonyms :
Burst promoting activity antibody Colony stimulating factor multiple antibody Eosinophil colony stimulating factor antibody Hematopoietic growth factor antibody IL 3 antibody IL-3 antibody IL3 antibody Interleukin 3 (colony stimulating factor, multiple) antibody Interleukin 3 antibody Interleukin-3 antibody Mast cell growth factor antibody MCGF antibody MGC79398 antibody MGC79399 antibody Multi CSF antibody Multilineage colony stimulating factor antibody Multipotential colony stimulating factor antibody Multipotential colony-stimulating factor antibody OTTHUMP00000065963 antibody P cell stimulating factor antibody P-cell-stimulating factor antibody Thy1 inducing factor antibody
Uniprot ID :
P08700
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company information

Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com925.485.4527
headquarters: USA
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