Anti-rat Growth Hormone Antibody | Boster Immunoleader RP1007 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-rat Growth Hormone Antibody

catalog :

RP1007

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat

application :

western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-rat Growth Hormone Antibody

Catalog Number :

RP1007

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat

Applications :

WB,IHC-P,ELISA,Neu,IP

Western blot :

Concentration: 0.1-0.5μg/ml; Species: Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Species: Rat

ELISA :

Concentration: 0.1-0.5μg/ml; Species: Rat

Neutralization :

Species: Rat

Immunoprecipitation :

Species: Rat

Notes :

By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E. coli-derived rat GH recombinant protein(Position: F27-F216).

Contents :

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Growth Hormone(GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98% amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.

Reference :

1. Niall, H. D.; Hogan, M. L.; Sauer, R.; Rosenblum, I. Y.; Greenwood, F. C. : Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971. 2. Sundstrom, M.; Lundqvist, T.; Rodin, J.; Giebel, L. B.; Milligan, D.; Norstedt, G. : Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 angstrom resolution. J. Biol. Chem. 271: 32197-32203, 1996.

Gene Name :

GH1

Protein Name :

Somatotropin

Gene Full Name :

growth hormone 1

Synonyms :

gH antibody GH-N antibody GH1 antibody Growth hormone 1 antibody Growth hormone antibody hGH-N antibody IGHD1B antibody Pituitary growth hormone antibody Somatotropin antibody

Uniprot ID :

P01244

more info or order :

Boster Immunoleader product webpage