Anti-mouse TNF alpha Antibody | Boster Immunoleader RP1000 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-mouse TNF alpha Antibody

catalog :

RP1000

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse

application :

western blot, ELISA, immunoprecipitation, immunohistochemistry - paraffin section

publications citing this reagent :

Li X, Peng F, Xie C, Wu W, Han X, Chen L. (E)-3-(3,4-Dimethoxyphenyl)-1-(5-hydroxy-2,2-dimethyl-2H-chromen-6-yl)prop-2-en-1-one ameliorates the collagen-arthritis via blocking ERK/JNK and NF-κB signaling pathway. Int Immunopharmacol. 2013;17:1125-33 pubmed publisher
Xu Y, Zhou Y, Lin H, Hu H, Wang Y, Xu G. Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury. Int J Mol Med. 2013;31:555-60 pubmed publisher
Zhou C, Wan Y, Chu X, Song Z, Xing S, Wu Y, et al. Urotensin II contributes to the formation of lung adenocarcinoma inflammatory microenvironment through the NF-κB pathway in tumor-bearing nude mice. Oncol Lett. 2012;4:1259-1263 pubmed
Liu H, Gao Z, Cui S, Sun D, Wang Y, Zhao C, et al. Inhibition of intestinal adenoma formation in APC(Min/+) mice by Riccardin D, a natural product derived from liverwort plant Dumortiera hirsuta. PLoS ONE. 2012;7:e33243 pubmed publisher
Yu M, Wang Z, Fang Y, Xiao M, Yuan Z, Lu C, et al. Overexpression of vasostatin-1 protects hypoxia/reoxygenation injuries in cardiomyocytes independent of endothelial cells. Cardiovasc Ther. 2012;30:145-51 pubmed publisher
Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, et al. Salvianolic acid B attenuates brain damage and inflammation after traumatic brain injury in mice. Brain Res Bull. 2011;84:163-8 pubmed publisher
Zhao J, Chen H, Li Y. Protective effect of bicyclol on acute alcohol-induced liver injury in mice. Eur J Pharmacol. 2008;586:322-31 pubmed publisher
Huang J, Wang Y, Guo J, Lu H, Lin X, Ma L, et al. Radiation-induced apoptosis along with local and systemic cytokine elaboration is associated with DC plus radiotherapy-mediated renal cell tumor regression. Clin Immunol. 2007;123:298-310 pubmed

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-mouse TNF alpha Antibody

Catalog Number :

RP1000

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Mouse.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse

Applications :

WB,IHC-P,ELISA,Neu,IP

Western blot :

Concentration: 0.1-0.5μg/ml; Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Species: Mouse

ELISA :

Concentration: 0.1-0.5μg/ml; Species: Mouse

Neutralization :

Species: Mouse

Immunoprecipitation :

Species: Mouse

Notes :

By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E. coli-derived mouse TNF alpha recombinant protein(Position: L80-L235).

Contents :

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Reference :

1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985. 2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.

Gene Name :

TNF

Protein Name :

tumor necrosis factor

Gene Full Name :

tumor necrosis factor

Synonyms :

APC1 antibody APC1 protein antibody Cachectin antibody DIF antibody Differentiation inducing factor antibody Macrophage cytotoxic factor antibody MCF antibody Necrosin antibody TNF a antibody TNF alpha antibody TNF antibody TNF Macrophage Derived antibody TNF Monocyte Derived antibody TNF Superfamily Member 2 antibody TNF superfamily, member 2 antibody TNF, macrophage derived antibody TNF, monocyte derived antibody TNF-a antibody TNF-alpha antibody TNFA antibody TNFA_HUMAN antibody TNFSF2 antibody Tumor necrosis factor alpha antibody Tumor necrosis factor antibody Tumor necrosis factor ligand superfamily member 2 antibody Tumor Necrosis Factor Precursor antibody Tumor Necrosis Factor, Membrane Form antibody Tumor necrosis factor, soluble form antibody Tumour Necrosis Factor Alpha antibody

Uniprot ID :

P06804

more info or order :

Boster Immunoleader product webpage