product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-Frizzled homolog 1 Antibody
catalog :
PB9594
quantity :
100 ug/vial
price :
240 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
product information
Product Name :
Anti-Frizzled homolog 1 Antibody
Catalog Number :
PB9594
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Frizzled-1(FZD1) detection. Tested with WB, IHC-P in Human.
Price :
240 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human
Applications :
WB,IHC-P
Western blot :
Concentration: 0.1-0.6μg/ml; Tested Species: Human
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-2μg/ml; Tested Species: Human
Notes :
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen :
A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1 (369-400aa YIAGFLLEDRVVCNDKFAEDGARTVAQG TKKE), identical to the related mouse and rat sequences.
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Background :
Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.
Reference :
1. "Entrez Gene: FZD1 frizzled homolog 1 (Drosophila)". 2. Gazit A, Yaniv A, Bafico A, Pramila T, Igarashi M, Kitajewski J, Aaronson SA (Oct 1999). "Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response". Oncogene 18(44): 5959–66. 3. Sagara N, Toda G, Hirai M, Terada M, Katoh M (Nov 1998). "Molecular cloning, differential expression, and chromosomal localization of human frizzled-1, frizzled-2, and frizzled-7". Biochemical and Biophysical Research Communications 252 (1): 117–22.
Gene Name :
FZD1
Protein Name :
Frizzled-1
Gene Full Name :
frizzled class receptor 1
Synonyms :
Chfz-1 antibody Dfrizzled-1 antibody DKFZp564G072 antibody FLJ95923 antibody Frizzled 1 antibody Frizzled homolog 1 (Drosophila) antibody Frizzled homolog 1 antibody Frizzled-1 antibody Fz receptor 1 antibody Fz-1 antibody Fz1 antibody FZD1 antibody FZD1_HUMAN antibody FzE1 antibody hFz1 antibody Rfz1 antibody Wnt receptor antibody Xfrizzled-1 antibody
Uniprot ID :
Q9UP38
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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