product summary
request information :
company name :
Boster Immunoleader
product type :
antibody
product name :
Anti-DLD Antibody
catalog :
PB9579
quantity :
100 ug/vial
price :
240 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
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product information
Product Name :
Anti-DLD Antibody
Catalog Number :
PB9579
Clonality :
Polyclonal
Description short :
Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P in Human;Mouse; Rat.
Price :
240 USD
Ig type :
Rabbit IgG
Size :
100 ug/vial
Form :
Lyophilized
Specificity :
No cross reactivity with other proteins.
Reactivity :
Reacts with: human, mouse, rat
Applications :
WB,IHC-P
Western blot :
Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded S ... :
Concentration: 0.5-1μg/ml; Tested Species: Human
Notes :
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen :
E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification :
Immunogen affinity purified.
Reconstitution :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage :
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Background :
DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Reference :
1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993.
2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001.
3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991.
Gene Name :
DLD
Protein Name :
Dihydrolipoyl dehydrogenase, mitochondrial
Gene Full Name :
dihydrolipoamide dehydrogenase
Synonyms :
Diaphorase antibody Dihydrolipoamide dehydrogenase antibody Dihydrolipoyl dehydrogenase antibody Dihydrolipoyl dehydrogenase mitochondrial antibody dld antibody DLDH antibody DLDH_HUMAN antibody E3 antibody E3 component of pyruvate dehydrogenase antibody E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody GCSL antibody Glycine cleavage system L protein antibody Glycine cleavage system protein L antibody LAD antibody lipoamide dehydrogenase antibody Lipoamide reductase antibody Lipoyl dehydrogenase antibody mitochondrial antibody OTTHUMP00000206744 antibody OTTHUMP00000206746 antibody OTTHUMP00000206748 antibody OTTHUMP00000206749 antibody PHE 3 antibody PHE3 antibody
Uniprot ID :
P09622
more info or order :
company information

Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com925.485.4527
headquarters: USA
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