Anti-DDB1 Antibody | Boster Immunoleader PB9578 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-DDB1 Antibody

catalog :

PB9578

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-DDB1 Antibody

Catalog Number :

PB9578

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA damage-binding protein 1(DDB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. And this protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Reference :

1. "Entrez Gene: DDB1 damage-specific DNA binding protein 1, 127kDa". 2. Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S (Feb 1996). "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein". Genomics 29 (1): 62–9. 3. Seki N, Hayashi A, Hattori A, Kozuma S, Sasaki M, Suzuki Y, Sugano S, Muramatsu M, Saito T (Jan 2000). "cDNA cloning, tissue expression, and chromosomal assignment of a mouse gene, encoding a 127 kDa UV-damaged DNA binding protein which is defective in XPE cells". DNA Res 6 (5): 319–22.

Gene Name :

DDB1

Protein Name :

DNA damage-binding protein 1

Gene Full Name :

damage-specific DNA binding protein 1, 127kDa

Synonyms :

Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody UV-DDB1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCE antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody

Uniprot ID :

Q16531

more info or order :

Boster Immunoleader product webpage