Boster Immunoleader

antibody

Anti-Daxx Antibody

PB9550

100 ug/vial

240 USD

polyclonal

rabbit

human, rat

western blot, immunohistochemistry - paraffin section

Anti-Daxx Antibody

PB9550

Polyclonal

Rabbit IgG polyclonal antibody for Death domain-associated protein 6(DAXX) detection. Tested with WB, IHC-P in Human;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Daxx recombinant protein (Position: K56-R345). Human Daxx shares 88.3% and 88.6% amino acid (aa) sequence identity with mouse and rat Daxx, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

DAXX (death-domain associated protein) also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al. (1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

1. Chang, H. Y., Nishitoh, H., Yang, X., Ichijo, H., Baltimore, D. Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx. Science 281: 1860-1863, 1998. 2. Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., Strauss, J. F., III. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol. 16: 1289-1298, 1997. 3. Yang, X., Khosravi-Far, R., Chang, H. Y., Baltimore, D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 89: 1067-1076, 1997.

DAXX

Death domain-associated protein 6

death-domain associated protein

BING 2 antibody BING2 antibody CENP-C binding protein antibody DAP 6 antibody DAP6 antibody Daxx antibody DAXX antibody DAXX_HUMAN antibody Death associated protein 6 antibody Death domain associated protein 6 antibody Death domain associated protein antibody Death domain-associated protein 6 antibody EAP 1 antibody EAP1 antibody ETS1 associated protein 1 antibody ETS1-associated protein 1 antibody Fas binding protein antibody Fas death domain associated protein antibody Fas death domain-associated protein antibody hDaxx antibody MGC126245 antibody MGC126246 antibody

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