Anti-CYP1A1 Antibody | Boster Immunoleader PB9544 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CYP1A1 Antibody

catalog :

PB9544

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

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product information

Product Name :

Anti-CYP1A1 Antibody

Catalog Number :

PB9544

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cytochrome P450 1A1(CYP1A1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Background :

CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation.

Reference :

1. Beresford AP (1993). "CYP1A1: friend or foe?". Drug Metabolism Reviews 25 (4): 503–17. 2. Uno, S (2004). "Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible cytochrome P450 is more important than metabolic activation.". Mol Pharmacol 65 (5): 1225–37.

Gene Name :

CYP1A1

Protein Name :

Cytochrome P450 1A1

Gene Full Name :

cytochrome P450, family 1, subfamily A, polypeptide 1

Synonyms :

AHH antibody AHRR antibody Aryl hydrocarbon hydroxylase antibody CP 11 antibody CP11 antibody CP1A1_HUMAN antibody CYP 1 antibody CYP1 antibody CYP1A1 antibody CYPIA1 antibody Cytochrome P1 450 dioxin inducible antibody Cytochrome P1-450 antibody Cytochrome P1-450 dioxin-inducible antibody Cytochrome P450 1A1 antibody Cytochrome P450 family 1 subfamily A polypeptide 1 antibody Cytochrome P450 form 6 antibody Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody Cytochrome P450-C antibody Cytochrome P450-P1 antibody Flavoprotein-linked monooxygenase antibody Microsomal monooxygenase antibody P1 450 antibody P450 C antibody P450 form 6 antibody P450 P1 antibody P450DX antibody Xenobiotic monooxygenase antibody

Uniprot ID :

P04798

more info or order :

Boster Immunoleader product webpage