Anti-Cdk2 Antibody | Boster Immunoleader PB9534 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cdk2 Antibody

catalog :

PB9534

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cdk2 Antibody

Catalog Number :

PB9534

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Reference :

1. Demetrick, D. J., Zhang, H., Beach, D. H.Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes.Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Tsai, L.-H., Harlow, E., Meyerson, M.Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated P33 kinase.Nature 353: 174-177, 1991.

Gene Name :

CDK2

Protein Name :

Cyclin-dependent kinase 2

Gene Full Name :

cyclin-dependent kinase 2

Synonyms :

Cdc2 related protein kinase antibody cdc2-related protein kinase antibody Cdk 2 antibody CDK2 antibody CDK2_HUMAN antibody Cell devision kinase 2 antibody Cell division kinase 2 antibody Cell division protein kinase 2 antibody Cyclin dependent kinase 2 antibody cyclin dependent kinase 2-alpha antibody Cyclin-dependent kinase 2 antibody p33 protein kinase antibody p33(CDK2) antibody

Uniprot ID :

P24941

more info or order :

Boster Immunoleader product webpage