Anti-CDK1 Antibody | Boster Immunoleader PB9533 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CDK1 Antibody

catalog :

PB9533

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CDK1 Antibody

Catalog Number :

PB9533

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 1(CDK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Reference :

1. Draetta, G.; Piwnica-Worms, H.; Morrison, D.; Druker, B.; Roberts, T.; Beach, D.: Human CDC2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate. Nature 336: 738-744, 1988. 2. Nazarenko, S. A.; Ostroverhova, N. V.; Spurr, N. K. : Regional assignment of the human cell cycle control gene CDC2 to chromosome 10q21 by in situ hybridization. Hum. Genet. 87: 621-622, 1991. 3. Spurr, N. K.; Goodfellow, P. N.; Nurse, P.; Lee, M. : Assignment of the human homologue of the yeast cell cycle control gene CDC2 to chromosome 10. (Abstract) Cytogenet. Cell Genet. 46: 698, 1987.

Gene Name :

CDK1

Protein Name :

Cyclin-dependent kinase 1

Gene Full Name :

cyclin-dependent kinase 1

Synonyms :

Cdc 2 antibody Cdc2 antibody CDC28A antibody CDK 1 antibody CDK1 antibody CDK1_HUMAN antibody CDKN1 antibody CELL CYCLE CONTROLLER CDC2 antibody Cell division control protein 2 antibody Cell division control protein 2 homolog antibody Cell division cycle 2 G1 to S and G2 to M antibody Cell division protein kinase 1 antibody Cell Divsion Cycle 2 Protein antibody Cyclin Dependent Kinase 1 antibody Cyclin-dependent kinase 1 antibody DKFZp686L20222 antibody MGC111195 antibody p34 Cdk1 antibody p34 protein kinase antibody P34CDC2 antibody

Uniprot ID :

P06493

more info or order :

Boster Immunoleader product webpage