Boster Immunoleader

antibody

Anti-Cdc6 Antibody

PB9531

100 ug/vial

240 USD

polyclonal

rabbit

human, rat

western blot, immunohistochemistry - paraffin section

Anti-Cdc6 Antibody

PB9531

Polyclonal

Rabbit IgG polyclonal antibody for Cell division control protein 6 homolog(CDC6) detection. Tested with WB, IHC-P in Human;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication.

1. Gonzalez, S., Klatt, P., Delgado, S., Conde, E., Lopez-Rios, F., Sanchez-Cespedes, M., Mendez, J., Antequera, F., Serrano, M. Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Mimura, S., Seki, T., Tanaka, S., Diffley, J. F. X. Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control. Nature 431: 1118-1123, 2004. 3. Whitmire, E., Khan, B., Coue, M. Cdc6 synthesis regulates replication competence in Xenopus oocytes. Nature 419: 722-725, 2002.

CDC6

Cell division control protein 6 homolog

cell division cycle 6

Cdc 18L antibody Cdc 6 antibody CDC18 (cell division cycle 18, S.pombe, homolog) like antibody CDC18 (S.pombe) antibody Cdc18 related protein antibody CDC18(S.pombe) antibody Cdc18-related protein antibody Cdc18L antibody cdc6 antibody CDC6 cell division cycle 6 homolog antibody CDC6 related protein antibody CDC6-related protein antibody CDC6_HUMAN antibody Cdc6p antibody Cell Cycle Controller antibody CELL CYCLE CONTROLLER CDC6 antibody Cell division control protein 6 antibody Cell division control protein 6 homolog antibody Cell Division Cycle 18 antibody Cell division cycle 6 homolog (S. cerevisiae) antibody Cell division cycle 6 homolog antibody HsCDC 6 antibody HsCDC18 antibody HsCDC6 antibody p62 antibody p62(cdc 6) antibody p62(cdc6) antibody

Q99741